產品資訊

CHAI Sahara Multiplex qPCR Master Mix:qPCR-Real Time PCR試劑組

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型式類型:試劑耗材
應用分類:PCR/qPCR 相關
代理品牌:CHAI
[原廠連結]

產品說明:

傳統 SYBR Green 效果不佳?想得到敏感度更高的Melt and HRM analysis結果?
快來試試CHAI Green & Sahara Multiplex qPCR Master Mix !

  • 表現更甚傳統SYBR Green,不會抑制PCR反應
  • 可獲得更高敏感度的Melt and HRM analysis
  • GC Rich達70%表現仍佳
  • 試劑內含特殊Enhance,可有效抑制DNA萃取液中的PCR Inhibitor
  • 可進行Multiplex PCR (up to 4-plex )
  • 熱穩定性高,25 ºC下可放置3個月仍不影響表現 (50 ºC for 8 days, or 25 ºC for 3 months)

產品特色:

  • Multiplex up to four Real-Time PCR reactions with no effect on Cq’s
  • Extreme thermal stability: withstands three months at 25 ºC with no impact on efficiency
  • Overcomes inhibition in crude extracts and environmental samples
  • Universal mix runs fast protocols, amplifies GC-rich targets, and incorporates aptamer-based hot start and dUTP carry-over prevention systems
  • License-free for commercial or diagnostic use

產品使用說明:

詳見原廠產品使用手冊↓
CHAI qPCR試劑 使用手冊


實驗參考數據:

  • Dynamic Range 超廣且結果具高度線性↓
                           Ara h2 gene fragment                                           E = 99.96%, R2 = 1.000
Ara h2 gene fragment  Standard Curve

Dynamic range of 1010 is demonstrated while maintaining excellent efficiency and linearity using Sahara Multiplex qPCR Master Mix to amplify a 157 bp gene fragment on an Open qPCR instrument.


  • 任何檢體之實驗結果皆具高度一致性↓
                  Enterococcus gDNA                            mGAPDH gene fragmen
                 E = 101.8%, R2 = 0.999t                      E = 99.4%, R2 = 0.999
    Enterococcus gDNAmGAPDH gene fragment
                       Yeast gene fragment                          mActin gene fragment
                     E = 100.7%, R2 = 1.000                       E = 99.9%, R2 = 0.983
 
        Yeast gene fragmentmActin gene fragment

Four targets were amplified simultaneously using Sahara Multiplex qPCR Master Mix on a Bio-Rad CFX Touch instrument. 10-fold dilutions of all targets achieved excellent linearity & efficiency.


  • 絕佳穩定度↓
                       Ara h2 gene fragment                                Lambda gDNA
Ara h2 gene fragmentLambda gDNA

                mActin gene fragment                                 Enterococcus gDNA
mActin gene fragmentEnterococcus gDNA

Sahara Multiplex qPCR Master Mix was stored at 50 ºC, and a fourplex reactions were run daily on Bio-Rad CFX96 Touch instrument. No difference in Cq is observed throughout the trial.


  • 速度超快效率極佳↓
                   Lambda gDNA, 72 bp amplicon                             E = 97%, R2 = 0.996
Ara h2 gene fragment Standard Curve

A fast 19 minute protocol was run on an Open qPCR instrument, obtaining excellent linearity & efficiency. Protocol: Initial denaturing 30s @ 95 ºC, Cycle 30x: 3s @ 95 ºC, 14s @ 60 ºC, 5 C ºC/s ramp times.


 

規格內容:

Specification of Sahara Multiplex qPCR Master Mix
 Concentration  2X
 Hot Start Mechanism  Aptamer
 GC Content  40 – 70%
 Multiplex Targets  Up to 4
 Reaction Speed  Fast
 dUTP Carryover Prevention  Yes
 Visible Loading Dye  Yes
 Exonuclease Activity  5' → 3'
 Supported Probes  TaqMan/Hydrolysis, Molecular Beacon, Scorpions
 Supported Templates   Genomic DNA, cDNA, Plasmid DNA
 Recommended Reaction Volume  10 – 50 µL

Order information of Sahara Multiplex qPCR Master Mix
Cat. No. Quantity
 #R02210S 2 * 1ml
 #R02210M 5 * 1ml
#R02210L 2 * 5ml
 #R02210XL 5 * 5ml
#R02210B 1 * 50ml
 #R02210BL 5 * 50ml

訂購與聯絡方式:

歡迎隨時洽詢產品專員訂購或詢問產品相關資訊
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02-2741-8169 
office@pan-asia-bio.com.tw



更新日期:

2019年9月25日

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