- Dynamic Range 超廣且結果具高度線性↓
Dynamic range of 1010 is demonstrated while maintaining excellent efficiency and linearity using Sahara Multiplex qPCR Master Mix to amplify a 157 bp gene fragment on an Open qPCR instrument.
Enterococcus gDNA mGAPDH gene fragmen
E = 101.8%, R2 = 0.999t E = 99.4%, R2 = 0.999
E = 100.7%, R2 = 1.000 E = 99.9%, R2 = 0.983
Four targets were amplified simultaneously using Sahara Multiplex qPCR Master Mix on a Bio-Rad CFX Touch instrument. 10-fold dilutions of all targets achieved excellent linearity & efficiency.
Sahara Multiplex qPCR Master Mix was stored at 50 ºC, and a fourplex reactions were run daily on Bio-Rad CFX96 Touch instrument. No difference in Cq is observed throughout the trial.
A fast 19 minute protocol was run on an Open qPCR instrument, obtaining excellent linearity & efficiency. Protocol: Initial denaturing 30s @ 95 ºC, Cycle 30x: 3s @ 95 ºC, 14s @ 60 ºC, 5 C ºC/s ramp times.