產品資訊

CHAI Green qPCR Master Mix:qPCR-Real Time PCR試劑組

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型式類型:試劑耗材
應用分類:PCR/qPCR 相關
代理品牌:CHAI
[原廠連結]

產品說明:

傳統 SYBR Green 效果不佳?想得到敏感度更高的Melt and HRM analysis結果?
快來試試CHAI Green & Sahara Multiplex qPCR Master Mix !

  • 表現更甚傳統SYBR Green,不會抑制PCR反應
  • 可獲得更高敏感度的Melt and HRM analysis
  • GC Rich達70%表現仍佳
  • 試劑內含特殊Enhance,可有效抑制DNA萃取液中的PCR Inhibitor

產品特色:

  • Produce excellent melt curves & HRM results with the highly fluorescent Chai Green dye
  • Overcomes inhibition in crude extracts and environmental samples
  • Universal mix runs fast protocols, amplifies GC-rich targets, and incorporates aptamer-based hot start and dUTP carry-over prevention systems
  • License-free for commercial or diagnostic use

產品使用說明:

  詳見原廠產品使用手冊↓
Chai Green qPCR Master Mix Manual



實驗參考數據:

  • Dynamic Range 超廣且結果具高度線性↓
                               Ara h2 gene fragment                                      E = 101.5%, R2 = 0.999
Ara h2 gene fragment Standard Curve

Dynamic range of 1010 is demonstrated while maintaining excellent efficiency and linearity using Chai Green qPCR Master Mix to amplify a 157 bp gene fragment on an Open qPCR instrument.


  • 混和相似檢體進行實驗仍可得清晰分明之Peak及Tm值↓
Clear Peaks

Reactions containing mActin, mActin + Lambda, and Lambda + mGAPDH targets were amplified using Chai Green qPCR Master Mix on an Open qPCR instrument. Peaks can clearly be identified and differentiated, even when multiple targets are present.


  • HRM分析結果完美呈現↓
Definitive HRM

A class 4 beta globin SNP (rs7936221) was genotyped using Chai Green qPCR Master Mix. Wild type, homozygous, and heterozygous variants were easily differentiated via HRM, despite the class 4 SNP’s Tm difference of only 0.2 ºC.


  • 速度超快效率極佳↓
                         Lambda gDNA, 72 bp amplicon                                E = 106%, R2 = 0.996
Ara h2 gene fragment Standard Curve

A fast 19 minute protocol was run on an Open qPCR instrument, obtaining excellent linearity & efficiency. Protocol: Initial denaturing 30 s @ 95 ºC, Cycle 30x: 3 s @ 95 ºC, 14 s @ 60 ºC, 5 C ºC/s ramp times.


 

規格內容:

Specification of CHAI Green qPCR Master Mix
Concentration 2X
Hot Start Mechanism Aptamer
GC Content 40 – 70%
Multiplex Targets Up to 2
Reaction Speed Fast
dUTP Carryover Prevention Yes
Visible Loading Dye Yes
Exonuclease Activity 5' → 3'
Supported Templates Genomic DNA, cDNA, Plasmid DNA
Recommended Reaction Volume 10 – 50 µL

† user must add UNG to enable


Order information of CHAI Green qPCR Master Mix
Cat. No. Quantity
 #R02201S 2 * 1ml
 #R02201M 5 * 1ml
#R02201L 2 * 5ml
 #R02201XL 5 * 5ml
#R02201B 1 * 50ml
 #R02201BL 5 * 50ml

訂購與聯絡方式:

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02-2741-8169 
office@pan-asia-bio.com.tw



更新日期:

2019年9月25日

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