- Dynamic Range 超廣且結果具高度線性↓
Dynamic range of 1010 is demonstrated while maintaining excellent efficiency and linearity using Chai Green qPCR Master Mix to amplify a 157 bp gene fragment on an Open qPCR instrument.
Reactions containing mActin, mActin + Lambda, and Lambda + mGAPDH targets were amplified using Chai Green qPCR Master Mix on an Open qPCR instrument. Peaks can clearly be identified and differentiated, even when multiple targets are present.
A class 4 beta globin SNP (rs7936221) was genotyped using Chai Green qPCR Master Mix. Wild type, homozygous, and heterozygous variants were easily differentiated via HRM, despite the class 4 SNP’s Tm difference of only 0.2 ºC.
A fast 19 minute protocol was run on an Open qPCR instrument, obtaining excellent linearity & efficiency. Protocol: Initial denaturing 30 s @ 95 ºC, Cycle 30x: 3 s @ 95 ºC, 14 s @ 60 ºC, 5 C ºC/s ramp times.